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W/Adreno-EGL( 1029):

I/Choreographer( 1368): Skipped 362 frames! The application may be doing too much work on its main thread.

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Cerebellar cortical lamination and foliation require ...

Jan 15, 2014· EGL area is proportional to CGNP proliferation (Heine and Rowitch, 2009) and was dramatically reduced in nestin-cre, A1 −/− A2 fl/fl mice. To test grossly for postnatal expansion of the EGL, we measured total EGL cross-sectional area at P0 and P7 in control and nestin-cre, A2 fl/fl mice.

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Showing 152 changed files with 1,099 additions and 15,381 deletions.

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D/dalvikvm( 74): GC_EXPLICIT freed 31K, 5% free 750K/788K, paused 1ms+1ms, total 10ms D/TextLayoutCache( 74): Using debug level = 0 - Debug Enabled = 0

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The DNA-repair protein AlkB, EGL-9, and leprecan define ...

Feb 19, 2001· The EGL-9 protein from Caenorhabditis elegans is necessary for normal muscle function, and its inactivation results in strong resistance to paralysis induced by the Pseudomonas aeruginosa toxin . We predict that EGL-9 is a novel hydroxylase that could elicit its action through the modification of sidechains of intracellular proteins.

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EGL thickness was measured on five alternative 5-μm paraffin sections from the medial region of E18.5 developing cerebella. Regional measurements of the EGL were determined by dividing the EGL into three regions, anterior, dorsal, and posterior, according to the anatomical positions. At least three animals were analyzed for each genotype.

Influence of orange juice over the genotoxicity induced by ...

Jun 14, 2005· Methyl methanesulfonate (MMS) and cyclophosphamide (CP) are alkylating agents that differ in their mode of action. MMS is a directly-acting, monofunctional agent, while CP is a bifunctional agent that requires metabolic activation to a reactive metabolite.

The DNA dioxygenase ALKBH2 protects Arabidopsis thaliana ...

The most obvious explanation for the MMS sensitive phenotype of the alkbh2-1 plants is that unrepaired, MMS-induced lesions block DNA replication and, thereby, cell growth. Thus, we investigated the levels of genomic 1-meA in wild-type and alkbh2-1 plants after exposure to MMS.

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Influence of orange juice over the genotoxicity induced by ...

At 24 h, MMS and CP were genotoxic according to both parameters evaluated in comparison to both the water and the orange juice treatments . A reduction in DNA damage was observed for both MMS and CP at 48 h. However, the reduction was significant only in DI for CP and in DF for MMS (P ≤ 0.05).

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The DNA-repair protein AlkB, EGL-9, and leprecan define ...

Feb 03, 2001· The EGL-9 protein from Caenorhabditis elegans is necessary for normal muscle function and its inactivation results in resistance against paralysis induced by the Pseudomonas aeruginosa toxin. EGL-9 and leprecan are predicted to be novel protein hydroxylases that might be involved in the generation of substrates for protein glycosylation.

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Jan 24, 2012· After 10 h of incubation at 37°C the plaques were counted to calculate PFU (Plaque Forming Units per ml). For phage modification, M13 or MS2 suspensions in C salts were treated with 15 mM MMS or 20 mM chloroacetaldehyde (CAA) for 30 min at 30°C. The reaction was stopped by dilution of the mixtures and PFU was estimated as described above.

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Oxidative demethylation by Escherichia coli AlkB directly ...

Sep 12, 2002· A 41-base oligonucleotide (TTTTTT(ATTTTTT) 5) was treated with 50 mM DMS in 75 mM sodium cacodylate, pH 7.4, at 30 °C, twice for 2 h, and then four times for 1 h…

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Repair deficient mice reveal mABH2 as the primary ...

May 17, 2006· In wild-type cells exposed to 2 mM MMS for 1 h, the amount of 1meA present was reduced by more than 50% after 8 h; approximately 10% of the induced 1meA remained in the cells after 36 h (Figure 4A and B). Such repair kinetics are comparable to those observed for other base lesions (Klungland et al, 1999).

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After overnight culture, 5 ml LB medium was diluted in 500 ml LB medium for shaking at 37°C for 2–3 h until OD 600 of 0.8–1.0 was reached. 0.2 mM IPTG was added to induce GST protein production for 4 h before harvesting pellets. Bacteria lysates were disrupted by the nanodebee homogenizer.

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